Changes in Retinal N-Acylethanolamines and their Oxylipin Derivatives During the Development of Visual Impairment in a Mouse Model for Glaucoma

Neurons are especially susceptible to oxidative damage, which is increasingly implicated in neurodegenerative disease. Certain N‐acylethanolamines (NAEs) have been shown to protect neurons from oxidative stress. Since glaucoma may be considered a neurodegenerative disorder and the survival of retinal neurons could also be influenced by N‐acylethanolamines, our goal was to quantify changes in certain N‐acylethanolamine species and their oxylipin derivatives in the retina of a mouse model for glaucoma. We also sought to identify relationships between these and parameters of glaucoma disease development, specifically intraocular pressure, visual acuity, and contrast sensitivity. Five N‐acylethanolamine species and three NAE oxylipin derivatives were quantified in retina from young and aged DBA/2Crl mice. N‐Acylethanolamines and NAE‐oxylipins in retinal extracts were quantified against deuterated standards by isotope dilution gas chromatography–mass spectrometry. Levels (nmol/g dry weight) of N‐arachidonoylethanolamine (anandamide; NAE 20:4) were significantly (p = 0.008) decreased in aged (2.875 ± 0.6702) compared to young animals (5.175 ± 0.971). Conversely, the anandamide oxylipin, 15(S)‐HETE ethanolamide (15(S)‐HETE EA), was significantly (p = 0.042) increased in aged (0.063 ± 0.009) compared to young animals (0.039 ± 0.011). Enzymatic depletion of the anandamide pool by 15‐lipoxygenase and consequent accumulation of 15(S)‐HETE ethanolamine may contribute to decreased visual function in glaucomatous mice. Since N‐acylethanolamines effectively attenuate glaucoma pathogenesis and associated visual impairment, our data provides additional rationale and novel targets for glaucoma therapies.     

What matters the most for economic development? Evidence from the Community Development Financial Institutions Fund

Governments use incentive programmes to attract new businesses. Due to limited resources, there are questions about which incentives are most effective. Using establishment data from California, and data on the location of disbursements through two federal programmes, we consider the effectiveness of different programmes in attracting new businesses. We find that the amount of funding through the New Markets Tax Credit programme has a significant impact on business activity but find no evidence of an effect from the Community Development Financial Institutions programme. Our results suggest that the effectiveness of these programmes depends on how they provide assistance.     

The Effects of Isolation and Acid Mine Drainage on Fish and Macroinvertebrate Communities of Monday Creek, Ohio, USA

Isolated headwater streams in mined watersheds may have good water quality and fish habitat, yet be disconnected from immigration sources by stream segments impaired by acid mine drainage (AMD). Studies of fish and macroinvertebrate communities, habitat, and a number of hydrochemical parameters in Monday Creek, Ohio, show that AMD eliminates fish communities and severely limits macroinvertebrate communities in directly affected tributaries. Isolated headwaters in the heavily mined Monday Creek watershed have relatively good water quality and habitat, but poor fish communities. Comparison of isolated Monday Creek headwaters with non-isolated reaches in unmined watersheds indicates that differences in fish communities are attributable to isolation. Fish communities in isolated headwaters have lower Indices of Biotic Integrity (IBI) than comparable non-isolated communities, reduced species numbers, and lower numbers of individuals, despite suitable habitat as measured by the Qualitative Habitat Evaluation Index (QHEI). Comparison of macroinvertebrate communities shows higher Invertebrate Community Indices (ICI), and no apparent species loss, which can be attributed to the obligate flight stage in the life cycle of many macroinvertebrates, which enables them to overcome aquatic barriers. The implication of this research is that there is an opportunity for recovery of depleted fish communities in large AMD-isolated areas with good water quality, suitable habitat, and intact macroinvertebrate communities, by downstream treatment or source control of AMD to create aquatic corridors for fish immigration.     

Evaluating the role of ivy (Hedera helix) in moderating wall surface microclimates and contributing to the bioprotection of historic buildings

The role of ivy (Hedera helix L.) on building walls is much debated, with arguments being put forward for it playing a biodeteriorative role (for example through ivy rootlets exploiting cracks and holes) as well as suggestions that it might provide some bioprotection (for example by the ivy canopy protecting the walls from other agents of deterioration such as frost). We have carried out a year-long study of the influence that ivy canopies play on wall surface microclimates at five sites across a range of climatic settings within England, using iButtons to monitor temperature and relative humidity fluctuations at the wall surface on ivy-covered and exposed walls. Hourly data illustrates a general mediating effect of ivy canopies on both temperature and relative humidity regimes. The ivy reduces extremes of temperature and relative humidity, with the most clearcut differences for temperature. Across all five sites the average daily maximum temperature was 36% higher and the average daily minimum temperature 15% lower on exposed vs ivy-covered surfaces. Differences in the exposure level of studied walls (i.e. whether they are shaded or not by trees or other walls) influenced the degree of microclimatic alteration provided by the ivy canopy. Other important factors influencing the strength of the ivy impact on microclimate were found to be thickness of the canopy and aspect of the wall. A detailed analysis of one site, Byland in North Yorkshire, illustrates the seasonal differences in impact of ivy on microclimates, with insulation against freezing being the dominant effect in January, and the removal of high temperature ‘spikes’ the dominant effect in July. The observed moderating role of ivy canopies on wall surface microclimates will reduce the likelihood of frost and salt deterioration to the building materials, thus contributing to their conservation. Further research needs to be done on other potentially deteriorative roles of ivy before an overall bioprotective role can be assumed, but the significant impact of ivy on wall surface microclimates across England is clear.     

Phage‐mediated transfer of virulence genes

Bacteriophages as accessory genetic elements play a crucial role in the dissemination of genes and the promotion of genetic diversity within bacterial populations. Such horizontal transfer of DNA is critical in the emergence of new pathogenic organisms, through the dissemination of genes encoding virulence factors such as toxins, adhesins and agressins. Phages can transfer genes that are not necessary for bacteriophage persistence and are generally recognised by their ability to convert their host bacteria to new phenotypes. This phenomenon is known as phage conversion. If such converting genes encode for virulence factors, the consequences of phage infection may include increased virulence of the host bacteria, and the conversion of a non‐pathogenic strain to a potentially dangerous pathogen. A number of virulence factors in bacteria causing diseases in plants, animals and humans are encoded by converting phages, the vast majority of which are temperate as opposed to lytic in nature. © 2001 Society of Chemical Industry     

Amphiphilic Interpenetrating Networks for the Delivery of Hydrophobic, Low Molecular Weight Therapeutic Agents

To investigate the delivery of hydrophobic therapeutic agents, a novel class of interpenetrating networks (IPNs) were synthesized and composed of two networks: methacrylic acid grafted with poly(ethylene glycol) tethers, P(MAA-g-EG), and poly(n-butyl acrylate) (PBA). The hydrophilic P(MAA-g-EG) networks are pH-responsive hydrogels capable of triggered release of an encapsulated therapeutic agent, such as a low molecular weight drug or a protein, when it passes from the stomach (low pH) to upper small intestine (neutral pH). PBA is a hydrophobic homopolymer that can affect the IPN swelling behavior, the therapeutic agent loading efficiencies in IPNs, and solute release profiles from IPNs. In dynamic swelling conditions, IPNs had greater swelling ratios than P(MAA-g-EG), but in equilibrium swelling conditions the IPN swelling ratio decreased with increasing PBA content. Loading efficiencies of the model therapeutic agent fluorescein ranged from 21 - 44%. Release studies from neat P(MAA-g-EG) and the ensuing IPNs indicated that the transition from low pH (2.0) to neutral pH (7.0) triggered fluorescein release. Maximum fluorescein release depended on the structure and hydrophilicity of the carriers used in these studies.     

Rating formats and rater training redux: A context-specific approach for enhancing the effectiveness of performance management.

In this paper, we critically examine previous research on rating formats and rater training in the context of performance appraisal. Historically, the goal of this body of research has been to search for ways of maximizing the psychometric quality of performance evaluation data. Our central thesis is that there are a number of avenues for broadening this research. Accordingly, we propose a conceptual model that hopefully serves as a conceptual framework for future work in these 2 traditional performance appraisal research streams. For example, both rating formats and rater training research may be useful for facilitating and improving the feedback and employee development process, as well as reducing the potential existence of rater biases. In addition, format and training research may focus upon ways of enhancing both rater and ratee reactions to the appraisal system. A key feature of our model is the integration of national culture as a moderator of the relations between specific formats, training programs, and various outcomes. We consider both the national culture of raters and ratees, and focus specifically on comparisons between Western and East Asian cultures. (PsycINFO Database Record (c) 2010 APA, all rights reserved)